International Collaborative Partner (ICP)

Name Prof. Dr. Chong Kowit-Yu

Ph.D. of Pharmacology, Southern Illinois University School of Medicine, Department of Pharmacology. Springfield, IL, USA (1999)

Bachelor of Science of Medical Technology
National Yang-Ming Medical University School of Medical Technology. Taipei, Taiwan (1988)

Organization Chang Gung University
UTAR Contact Person Prof Dr Alan Ong Han Kiat,

Aug 2018 - Present Professor, Department of Medical Biotechnology and Laboratory Science, Graduate Institute of Medical Biotechnology, Chang Gung University, Taoyuan, Taiwan
Aug 2015- July 2018 Associate Professor, Department of Medical Biotechnology and Laboratory Science, Graduate Institute of Medical Biotechnology, Chang Gung University, Taoyuan, Taiwan
Oct 2004- July 2015 Assistant Professor, Department of Medical Biotechnology and Laboratory Science, Graduate Institute of Medical Biotechnology, Chang Gung University, Taoyuan, Taiwan
June, 2003-July 2004 Visiting Assistant Professor, Graduate Institute of Medical Biotechnology, Chang Gung University, Taoyuan, Taiwan
Nov 2002- Sept 2004 Assistant Investigator, Pittsburgh Development Center (PDC), Magee-Womens Research Institute, Pittsburgh, PA, USA
Sept 2001-Sept 2004 Research Instructor, Dept Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.
Teaching & Administration
  • Introduction to Biotechnology
  • Fundamental Biotechnology
  • Biotechnology Laboratory
  • Advanced Biotechnology
  • The Frontier of Biotechnology in Molecular Diagnosis Laboratory
  • Recent advances in Biotechnology Applied in human Diseases
  • Stem Cell Biology
  • Frontiers Regenerative Medicine
  • Tissue Engineering
  • Embryonic Stem Cell Culture Techniques Laboratory
  • Advanced Embryonic Germ Cell Culture Techniques
  • Differentiation of Hematopoietic Progenitor Cells Laboratory
Teaching Interests
  • Biotechnology
  • Stem Cell
  • Tissue Engineering
Research Interests
  • Stem cells and lung related diseases
  • Multiple drug resistant cancer cells and drug development
Funded Research Projects
  • Investigation of the protective effects of hypoxia preconditioned mesenchymal stem cells against Bleomycin-induced Pulmonary Fibrosis in mouse model.
  • Investigation of the Relationship between Drug Resistance and Wnt pathway in Multiple Drug Resistant Cancer Cell line
Awards & Honours
  • Excellent Teacher Award in Student Counseling", Chang Gung University, Tao-Yuan, Taiwan (September, 2011)
  • Travel Poster Award - Gordon Research Conference-Multi-Drug Efflux Systems. Les Diablerets, Switzerland (June, 2011)
  • Excellent Research Poster Award - International Symposium and Workshop on Taiwan Medicinal mushroom: Antrodio cinnamomea. Academia Sinica, Taipei, Taiwan (May, 2014).
  • React-the-World Travel Awards - XXVI Congress of the International Society on Thrombosis and Haemostasis and 63rd Annual Scientific and Standardization Committee (SSC). Berlin, Gemany (July 2017)
Publications & Presentations
1. Choo KB, Lee HH, Liew LN, Chong KY, Chou HF. Analysis of the unoccupied site of an integrated human papillomavirus 16 sequence in a cervical carcinoma. Virology 178: 621-625, 1990.
2. Choo KB, Liew LN, Chong KY, Lu RH, Cheng WT. Transgenome transcription and replication in the liver and extrahepatic tissues of a human hepatitis B virus transgenic mouse. Virology 182: 785-792, 1991.
3. Choo KB, Chong KY, Liew LN, Hsu HC, Cheng WT. Unregulated and basal transcriptional activities of the regulatory sequence of the type 18 human papillomavirus genome in transgenic mice. Virology 188: 378-383, 1992.
4. Choo KB, Chong KY. Absence of mutation in the p53 and the retinoblastoma susceptibility genes in primary cervical carcinomas. Virology 193: 1042-1046, 1993.
5. Chong KY, Chen CM, Choo KB. Post-hybridization recovery of membrane filter-bound DNA for enzymatic DNA amplification. Biotechniques 14: 575-578, 1993.
6. Su C-Y, Chong KY, Owen OE, Dillmann WH, Chang C, Lai C-C. Constitutive and inducible HSP70s are involved in oxidative resistance evoked by heat shock or ethanol. J Mol Cell Cardiol 30: 587-598, 1998.
7. Chong KY, Lai C-C, Lille S, Chang C and Su C-Y. Stable Overexpression of the Constitutive Form of Heat Shock Protein 70 Confers Oxidative Protection. J Mol Cell Cardiol 30: 599-608, 1998.
8. Su C-Y, Chong KY, Chen JX, Ryter S, Lai C-C. A Physiologically Relevant Hyperthermia Selectively Avtivates Constitutive hsp70 in H9c2 Cardiac Myoblasts and Confers Oxidative Protection J Mol Cell Cardiol 31: 845-855, 1999.
9. Su C-Y, Chong KY, Edelstein KM, Lille S, Khardori R, Lai C-C. Costitutive hsp70 Attenuates Hydrogen Peroxide-induced Mmembrane Lipid Peroxidation. Biochem Biophys Res Commun 265; 279-284, 1999.
10. Chan AWS, Chong KY, Martinovich C, Simerly C, Schatten G. Transgenic monkeys produced by retroviral gene transfer into mature oocytes. Science 291, 309-312, 2001.
11. Cabot RA, Kuhholzer B, Chan AWS, Lai L, Park KW, Chong KY, Schatten G, Murphy CN, Abeydeera L, Day BN and Prather RS. Transgenic Pigs produced using In Vitro matured Oocytes infected with a Retroviral Vector. Animal Biotechnology 12: 205-214, 2001.
12. Chan AWS, Chong KY and Schatten G. P. Transgenic bovine embryo selection using green fluorescent protein. Method in Molecular biology 183: 201-214, 2002.
13. Simerly C, Dominko T, Navara C,Payne C, Capuano S, Gosman G, Chong KY, Khorram O, Jones J, Mountain V, Compton D, Hewitson L and Schatten G. Primate Nuclear Transfer Failures: Defective Centrosomes Organize Faulty Mitotic Spindles Producing Aneuploid Embryos. Science 300: 297, 2003
14. Simerly C, Navara C, Hyun SH, Lee BC, Kang SK, Capuano S, Gosman G, Dominko T, Chong KY, Compton D, Hwang WS, & Schatten G. Embryogenesis And Blastocyst Development After Somatic Cell Nuclear Transfer In Non-Human Primates (Nhp-Scnt): Overcoming Defects Caused By Meiotic Spindle Extraction. Dev Biol 276: 237-252, 2004.
15. Tseng CP, Huang CL, Chong KY, Hung IJ, Chiu DT. Rapid detection of glucose-6-phosphate dehydrogenase gene mutations by denaturing high-performance liquid chromatography. Clin Biochem, 38: 973-980, 2005.
16. Choo KB, Hsu MC, Chong KY, Huang CJ. Testis-specific expression and genomic multiplicity of the rat Rtdpoz genes that encode bipartite TRAF- and POZ/BTB-domain proteins. Gene, 387: 141-149, 2007.
17. Liu FC, Chen HL, Chong KY, Hsu AL and Chen CM. Production of recombinant porcine colipase secreted by Pichia Patoris and its application to improve dietary fat digestion and growth of postweaning Piglets, Biotechnol. Prog, 24, 1333-1341, 2008.
18. Yen CC*, Lin CY*, Chong KY* Tsai TC, Shen CJ, Lin MF, Su CY, Chen HL and Chen CM. Lactoferrin as a natural regimen of selective decontamination of the digestive tract: recombinant porcine Lactoferrin expression in the milk of transgenic mice protects neonates from pathogen challenges in the gastrointestinal tract. J Inf Dis. 199:590-598, 2009. *These authors make equal contribution to this paper.
19. Tseng WL, Huang CL, Chong KY, Liao CH, Stern A, Cheng JC, Tseng CP. Reelin is a platelet protein and functions as a positive regulator of platelet spreading on fibrinogen. Cell Mol Life Sci. 67: 641-653, 2010.
20. Huang CL, Cheng JC, Kitajima K, Nakano T, Chong KY#, Tseng CP. Disabled-2 is required for mesoderm differentiation of murine embryonic stem cells. . J Cell Physiol. 2010 225:92-105, 2010. (co-corresponding author)
21. Tsai TC, Lin W, Yang SH, Cheng WT, Cheng EH, Lee MS, Chong KY, Chen CM. Granzyme G is expressed in the two-cell stage mouse embryo and is required for the maternal-zygotic transition. BMC Dev Biol. 2011. [Article ID 238208].
22. Hung CM*, Yeh CC, Chong KY*, Chen HL*, Chen JY, Kao ST, Yen CC, Yeh MH, Lin MS, Chen CM. Gingyo-San Enhances Immunity and Assists Infectious Bursal Disease Vaccination. Evidence-based Compl. and Alt. Medicine 2011: 238208, 2011 *These authors make equal contribution to this paper.
23. Chong KY#, Lai CC, Su CY. Inducible and Constitutive HSP70s confer a synergistic resistance against oxidative insults. Biochem Biophys Res Commun. 430: 774-779, 2013.
24. Huang TT, Chong KY, Ojcius DM, Wu YH, Ko YF, Wu CY, Martel J, Lu CC, Lai HC, Young JD. Hirsutella sinensis mycelium suppresses interleukin-1β and interleukin-18 secretion by inhibiting both canonical and non-canonical inflammasomes. Sci Rep. 3:1374. doi: 10.1038/srep01374, 2013.
25. Chen W, Chen JY, Tung YT, Chen HL, Kuo CW, Chuang CH, Chong KY, Mao FC, Chen CM. High-frequency ultrasound imaging to evaluate liver fibrosis progression in rats and yi guan jian herbal therapeutic effects. Evid Based Complement Alternat Med. 2013:302325, 2013.
26. Huang TT, Lai HC, Chen YB, Chen LG, Wu YH, Ko YF, Lu CC, Chang CJ, Wu CY, Martel J, Ojcius DM, Chong KY, Young JD. Cis-resveratrol suppresses IL-1β secretion by inhibiting the non-canonical inflammasome pathway in macrophages. Innate Immunity, 20: 735-750, 2014.
27. Tung YT, Tang TY, Chen HL, Yang SH, Chong KY, and Chen CM. Lactoferrin protects against chemical-induced rat liver fibrosis through inhibiting stellate cells activation. J. Dairy Sci. J Dairy Sci. 97: 3281-91, 2014.
28. Lai CW, Chen HL, Lin KY, Liu FC, K. Y. Chong KY, Cheng WT and Chen CM. 2014. FTSJ2, a heat shock-inducible mitochondrial protein, suppresses cell invasion and migration. PLoS One. 9: e90818, 2014.
29. Huang TH, Chen CM, Tseng CP, Shen CJ, wang TH, Choo, KB, Chong KY#. FZD1 activates Protein Kinase C delta-mediated drug-resistance in multidrug-resistant cancer cells. Int J Biochem Cell Biol, 53: 55-65, 2014.
30. Hung TH, Hsu SC, Cheng CY, Choo KB, Tseng CP, Chen TC, Lan YW, Huang TT, Lai HC, Chen CM, Chong KY#. Wnt5A regulates ABCB1 expression in multidrug-resistant cancer cells through activation of the non-canonical PKA/β-catenin pathway. Oncotarget. 5: 12273-12290, 2014.
31. Huang TT, Wu SP, Chong KY, Ojcius DM, Ko YF, Wu YH, Wu CY, Lu CC, Martel J, Young JD, Lai HC. The medicinal fungus Antrodia cinnamomea suppresses inflammation by inhibiting the NLRP3 inflammasome. J Ethnopharmacol.155: 154-164, 2014.
32. Tsai TC, Tung YT, Kuo YH, Liao JW, Tsai HC, Chong KY, Chen HL, Chen CM. Anti-inflammatory effects of Antrodia camphorata, a herbal medicine, in a mouse skin ischemia model. J Ethnopharmacol. 159:113-121, 2015.
33. Chong KY#, Hsu CJ, Hung TH, Hu HS, Huang TT, Wang TH, Wang CH, Chen CM, Choo KB, and Tseng CP. Wnt pathway activation and ABCB1 expression account for attenuation of Proteasome Inhibitor-mediated apoptosis in multidrugresistant cancer cells. Cancer Biol Ther. 16:149-159, 2015.
34. Hung TH, Li YH, Tseng CP. Lan YW, Hsu SC, Chen YH, Huang TT, Lai HC, Chen CM, Choo KB, Chong KY#. Knockdown of c-MET induced Apoptosis in ABCB1-Overexpressed Multidrug-Resistance Cancer Cell Lines. Cancer Gene Ther, 22:262-270, 2015.
35. Lin MH, Shu JC, Lin LP, Chong KY, Cheng YW, Du JF, Liu ST. Elucidating the crucial role of poly N-acetylglucosamine from Staphylococcus aureus in cellular adhesion and pathogenesis. PLoS One. 10:e0124216, 2015.
36. Chen YB, Lan YW, Hung TH, Chen LG, Choo KB, Cheng WT, Lee HS, Chong KY#. Mesenchymal stem cell-based HSP70 promoter-driven VEGFA induction by resveratrol promotes angiogenesis in a mouse model. Cell Stress Chaperones. 20:643-652, 2015.
37. Tung YT, Huang PW, Chou YC, Lai CW, Wang HP, Ho HC, Yen CC, Tu CY, Tsai TC, Yeh DC, Wang JL, Chong KY, Chen CM. Lung tumorigenesis induced by human vascular endothelial growth factor (hVEGF)-A165 overexpression in transgenic mice and amelioration of tumor formation by miR-16. Oncotarget. 6:10222-10238, 2015
38. Lan YW, Choo KB, Chen CM, Hung TH, Chen YB, Hsieh CH, Kuo HP, Chong KY#. Hypoxia-preconditioned mesenchymal stem cells attenuate bleomycin-induced pulmonary fibrosis. Stem Cell Res Ther. 6:97, 2015. doi:10.1186/s13287-015-0081-6.
39. Chen YB, Lan YW, Hung TH, Chen LG, Choo KB, Cheng WT, Lee HS, Chong KY#. Mesenchymal stem cell-based HSP70 promoter-driven VEGFA induction by resveratrol promotes angiogenesis in a mouse model. Cell Stress Chaperones. 20:643-652,2015.
40. Chen YB, Lan YW, Chen LG, Huang TT, Choo KB, Cheng WTK, Lee HS, Chong KY#. Mesenchymal stem cell-based HSP70 promoter-driven VEGFA induction by resveratrol alleviates elastase-induced emphysema in a mouse model. Cell Stress Chaperones. 20: 979-89, 2015.
41. Hsu CY, Shu JC, Lin MH, Chong KY, Chen CC, Wen SM, Hsieh YT, Liao WT. High Glucose Concentration Promotes Vancomycin-Enhanced Biofilm Formation of Vancomycin-Non-Susceptible Staphylococcus aureus in Diabetic Mice. PLoS One. 10:e0134852, 2015.
42. Huang TT, Lai HC, Ko YF, Ojcius DM, Lan YW, Martel J, Young JD, Chong KY#. Hirsutella sinensis mycelium attenuates bleomycin-induced pulmonary inflammation and fibrosis in vivo. Sci Rep. 5:15282. 2015 doi: 10.1038/srep15282.
43. Chang YW, Hsieh PW, Chang YT, Lu MH, Huang TF, Chong KY, Liao HR, Cheng JC, Tseng CP. Identification of a novel platelet antagonist that binds to CLEC-2 and suppresses podoplanin-induced platelet aggregation and cancer metastasis. Oncotarget. 6: 42733-42748, 2015.
44. Shih CL, Chong KY, Hsu SC, Chien HJ, Ma CT, Chang JW, Yu CJ, Chiou CC. Development of a magnetic bead-based method for the collection of circulating extracellular vesicles. N Biotechnol. 33:116-22. 2016.
45. Lai CW, Chen HL, Tsai TC, Chu TW, Yang SH, Chong KY, Chen CM. Sexually dimorphic expression of eGFP Transgene in the Akr1A1 Locus of mouse liver regulated by sex hormone-related epigenetic remodeling. Sci Rep. 6: 24023, 2016.
46. Chen YL, Hwang TL, Yu HP, Fang JY, Chong KY, Chang YW, Chen CY, Yang HW, Chang WY, Hsieh PW. Ilex kaushue and Its Bioactive Component 3, 5-Dicaffeoylquinic Acid Protected Mice from Lipopolysaccharide-Induced Acute Lung Injury. Sci Rep. 6: 34243, 2016.
47. Chang PY, Huang Y, Hung TY, Chong KY, Chang YS, Chao CC, Chow KN. Spontaneous metastases in immunocompetent mice harboring a primary tumor driven by oncogene latent membrane protein 1 from Epstein-Barr virus. Biomed J. 39: 261-271, 2016.
48. Lan YW, Theng SM, Huang TT, Choo KB, Chen CM, Kuo HP, Chong KY#. Oncostatin M-Preconditioned Mesenchymal Stem Cells Alleviate Bleomycin-Induced Pulmonary Fibrosis Through Paracrine Effects of the Hepatocyte Growth Factor. Stem Cells Transl Med. 6: 1006-1017, 2017.
49. Lai CW, Chen HL, Tu MY, Lin WY, R�hrig T, Yang SH, Lan YW, Chong KY#, Chen CM#. A novel osteoporosis model with ascorbic acid deficiency in Akr1A1 gene knockout mice. Oncotarget. 8: 7357-7369, 2017. #co-corresponding author
50. Chen YL, Hwang TL, Fang YF, Lan YH, Chong KY, Hsieh PW. Polysaccharides from Kochia scoparia fruits protect mice from lipopolysaccharide-mediated acute lung injury by inhibiting neutrophil elastase. J. Funct. Foods. 38: 582-590, 2017.
51. Tung YT, Chen HL, Wu HS, Ho MH, Chong KY, Chen CM. Kefir Peptides Prevent Hyperlipidemia and Obesity in High-Fat-Diet-Induced Obese Rats via Lipid Metabolism Modulation. Mol Nutr Food Res. 2018 Feb; 62(3). doi: 10.1002/mnfr.201700505. [Epub ahead of print].
52. Chang YW, Tseng CP, Lee CH, Hwang TL, Chen YL, Su MT, Chong KY, Lan YW, Wu CC, Chen KJ, Lu FH, Liao HR, Hsueh C, Hsieh PW. β-Nitrostyrene derivatives attenuate LPS-mediated acute lung injury via the inhibition of neutrophil-platelet interactions and NET release. Am J Physiol Lung Cell Mol Physiol. 314: L654-L669, 2018.
53. Huang TT, Lan YW, Ko YF, Chen CM, Lai HC, Ojcius DM, Martel J, Young JD, Chong KY#. Antrodia cinnamomea produces anti-angiogenic effects by inhibiting the VEGFR2 signaling pathway. J Ethnopharmacol.
54. Hiew MSY, Cheng HP, Huang CJ, Chong KY, Cheong SK, Choo KB, Kamarul T. Incomplete cellular reprogramming of colorectal cancer cells elicits an epithelial/ mesenchymal hybrid phenotype. J Biomed Sci. 25: 57, 2018.
Professional Membership
  • Taiwan Society of Stem Cell Research (TSSCR)
  • International Society for Stem Cell Research (ISSCR)
  • American Association for Cancer Research
Professional Activities
  • Editorial Board of Scientific Reports (Mar 2017-Present)